光合细菌和螺旋藻对黄泔水净化与利用初探

第20卷第4期1999年7月环境科学ENVIRONMENTALSCIENCEVol.20,No.4July,1999光合细菌和螺旋藻对黄泔水净化与利用初探郑爱榕1许伟斌2蔡阿根1方志山1(1.厦门大学亚热带海洋研究所,厦门361005,E-mail:YPHuang@jingxian.xmu.edu.cn2.厦门侨星饮料厂,厦门361005)摘要应用光合细菌将黄泔水中的有机物降解转化为无机氮磷后养殖螺旋藻.结果表明,在实验条件下光合细菌对黄泔水CODCr的去除率可达70%,处理后的黄泔水用甲壳质絮凝澄清后加入螺旋藻并曝气进行培养,藻生长周期为22d、生长速率为20～30mg/L·d.藻体蛋白质含量平均为52.6g/100g(干重),游离氨基酸平均含量为2.41g/100g(干重).关键词光合细菌,螺旋藻,黄泔水,净化与利用.郑爱榕:女,41岁,理学硕士,副教授收稿日期:1998-09-20TreatmentandReuseoftheWastewaterofBeanProductswiththePhytosyntheticBacteriaandSpirulinamaximaZhengAirong1XuWeibin2CaiAgen1FangZhishan1(1.InstituteofSubtropicalOceanogrophy,XiamenUniversity,Xiamen361005,ChinaE-mail:YPHuang@jinxian.xmu.edu.cn2.QiaoxingBeverageFactory,Xiamen361005)AbstractAtryonthetreatmentandreuseofthewastewaterofbeanproductswiththephytosyntheticbacteria(PSB)andS.maxima(SM)wasintroduced.FirstthewastewaterofbeanproductswastreatedwithPSBtode-compositingorganicmatter,andthenchitinwasaddedforprecipitatingPSB,thelastSMwasaddedintothetreatedwastewaterforremovingorganicmatterfurtherandreusingnitrogenandphosphorous.TheresultsofandynamicmodledexperimentshowedthatPSBhaveahighremovalefficiencyforCOD(70%)onthewastewa-terofbeanproducts,andSMculturedinthetreatedwastewastergrowwell,therateofgrowingis20～30mgdry-weight/(L·d),andthecontentofproteinandfreeaminoacidinSMare52.6g/100gdry-weightand2.41g/100gdry-weightrespectively.Keywordsphytosyntheticbacteria,Spirulinamaxima,wastewaterofbeanproducts,treamentandreuse.国内利用光合细菌(phytosyntheticbacte-ria,PSB)处理黄泔水已取得满意的结果[1],但处理的废水未加以利用.本文在光合细菌-螺旋藻体系处理与净化啤酒废水研究[2]的基础上,试图应用PSB将黄泔水中高浓度的有机大分子物质降解为无机的氮和磷后,用于养殖螺旋藻,以达到废水无害化和资源化的目的.1材料与方法1.1实验材料PSB:系沼泽红假单胞菌(Phodopseu-domonaspalustris),福建省水产研究所提供.培养液配方为:NH4Cl1.0g;NaAc3.5g;MgCl20.1g;CaCl20.1g;KH2PO40.6g;K2HPO40.4g;酵母膏0.1g;自来水1000ml.培养液115℃高温灭菌15～20min后调pH=7.0,28～30℃厌氧光照(25W白炽灯对称照明).极大螺旋藻(Spirulinamaxima):由厦门大学生物学系提供,采用CFTRI培养基[3],在28℃、1000lx、L∶D=12∶12的条件下纯种培养.黄泔水:取自厦门大学豆制品加工厂.其成分和主要含量(mg/L)分别为:CODCr16500;总磷26.8;总氮9.6;亚硝氮0.3;pH5.57.0.4%甲壳质:系商品级,用1%的醋酸溶液配制.*农牧渔业部螺旋藻工作组,江西省农科院情报所.蓝藻-螺旋藻(Spirulinapla