癌组织中CD45RO表达水平与肺腺癌患者临床病理特征及生存率的关联分析

·448··论著·癌组织中CD45RO表达水平与肺腺癌患者临床病理特征及生存率的关联分析俞达辉，吴道立，袁冲，褚文炎（余姚市人民医院心胸外科，浙江余姚315400）摘要目的评估肿瘤组织CD45RO表达水平与肺腺癌手术患者的临床病理参数及预后的相关性。方法选取121例进行手术的肺腺癌患者，采集肿瘤组织和癌旁组织样本。采用anti-CD45RO抗体进行免疫组织化学染色检测CD45RO表达水平，计算染色强度分数和染色面积分数的乘积得到总表达评分，并分为高、中和低表达。结果相对于癌旁组织，CD45RO在肺腺癌组织中高表达；肿瘤组织CD45RO表达水平与肿瘤大小、淋巴结转移呈负相关；Kaplan-Meier（K-M）曲线显示，肺腺癌组织中的CD45RO表达水平越高，无病生存期（DFS）和总体生存期（OS）越长；多因素COX回归发现，肺腺癌组织CD45RO表达量能独立预测良好的DFS，然而，低分化和淋巴结转移均能独立预测不良的DFS；肺腺癌组织CD45RO表达量是较长OS的独立预测因素，而低分化、淋巴转移及远端转移均是OS的独立危险因素。结论肿瘤组织中CD45RO的表达水平可作为新的、可靠的生物标记物，用于评估肺腺癌手术患者的临床病理特征和预后情况。关键词肺腺癌组织；CD45RO；临床病理特征；预后；肺腺癌手术患者中图分类号R734.2文献标志码A文章编号0258-4646（2018）05-0448-06网络出版地址DOI：10.12007/j.issn.0258‐4646.2018.05.015CorrelationofTissueCD45ROwiththeClinicopathologicalPropertiesandPrognosisinSurgicalPatientswithLungAdenocarcinomaYUDahui，WUDaoli，YUANChong，CHUWenyan（DepartmentofCardiothoracicSurgery，YuyaoPeople’sHospital，Yuyao315400，China）AbstractObjectiveToevaluatetheassociationbetweentumortissueCD45ROexpressionandtheclinicopathologicalpropertiesandprognosesinsurgicalpatientswithlungadenocarcinoma（LAC）.MethodsOnehundredandtwenty-onepatientswithLACwerere-cruitedforthestudy.LACtissuesandpairedadjacentLACtissuesampleswerecollected.CD45ROexpressionwasevaluatedbasedonimmunohistochemistry.ResultsCD45ROexpressionwashigherintumortissuesamplesthaninpairedadjacentLACtissuesamples.TumortissueCD45ROexpressionwaspositivelyassociatedwithtumorsizebutwasnegativelyassociatedwithlymphnodemetastasis（LYM）.Kaplan-MeiercurvesrevealedthathightumortissueCD45ROexpressionwascorrelatedwithbetterdisease-freesurvival（DFS）andoverallsurvival（OS）comparedtothoseoflowtumortissueCD45ROexpression.UnivariateCOXregressionindicatedthathightu-mortissueCD45ROexpressionpredictedprolongedDFS，whilepoordifferentiation，LYM，andtumor-node-metastasis（TNM）stagepre-dictedworseDFS.MultivariateCOXregressionrevealedthathightumortissueCD45ROexpressionwasanindependentprotectivefactorofDFS，whilepoordifferentiationandLYMactedasindependentriskfactorsofDFS.Furthermore，univariateanalysisindicatedthat