城市河道 污水 中好氧反硝化菌的分离鉴定与特性 (1)

微生物学通报APR20,2010,37(4):566572MicrobiologyChina©2010byInstituteofMicrobiology,CAStongbao@im.ac.cn基金项目：浙江省科技计划项目(No.2007C22032);浙江省农业科学院创新提升工程项目*通讯作者：Tel:86-27-87281040;:gxy@mail.hzau.edu.cn收稿日期：2009-01-22;接受日期：2010-02-09研究报告城市河道污水中好氧反硝化菌的分离鉴定与特性詹吉东1,2汤江武2王新2姚晓红2吴逸飞2周莉1葛向阳1*(1.华中农业大学生命科学与技术学院湖北武汉430070)(2.浙江省农业科学院植物保护与微生物研究所浙江杭州310021)摘要:采用富集培养和BTB(溴百里酚蓝)平板法从城市河道污水中筛选、分离获得了一株高效的好氧反硝化菌株ADZ1,48h内对硝酸盐的降解率为93.1%,总氮的去除率为34.7%。16SrRNA测序及系统发育分析结果表明该菌株属于Pseudamonassp.,经VITEK®2系统鉴定为Pseudomonasputida。对该菌株的反硝化特性进行了研究,结果表明,该菌株以乙醇为最佳碳源,在碳氮比达到12:1时,对硝酸盐的去除率达到98%以上,总氮去除率达到41.3%。该菌株对溶解氧、pH有着广泛的适应性,菌体活力强,有着良好的应用前景。关键词:好氧反硝化,城市河道,生物脱氮,恶臭假单胞菌(Pseudomonasputida)IsolationandCharacterizationofAerobicDenitrifierfromtheSewageofUrbanRiversZHANJi-Dong1,2TANGJiang-Wu2WANGXin2YAOXiao-Hong2WUYi-Fei2ZHOULi1GEXiang-Yang1*(1.CollegeofLifeScienceandTechnology,HuazhongAgriculturalUniversity,Wuhan,Hubei430070,China)(2.InstituteofPlantProtectionandMicrobiology,ZhejiangAcademyofAgriculturalScience,Hangzhou,Zhejiang310021,China)Abstract:AbacterialstrainADZ1thatdemonstratedhigherefficientintheaerobicdenitrificationwasiso-latedfromthesewageofurbanriversontheselectivemedium,BromothymolBlue(BTB),afterenrichedintheshakingflasks.Thereductionefficiencyfornitrateandthetotalnitrogenreached93.1%and34.7%within48hoursrespectively.Sequenceof16SrRNAsuggestedthatstrainADZ1belongstothegenusofPseudamonas,andfurtherwasidentifiedasPseudomonasputidabyVITEK®2systems.WiththegrowthconditionsthattheethanolservedasthecarbonsourceandC/Nratiowas12:1,theefficiencyfornitrateandthetotalnitrogenreductionreached98%and41.3%respectively.DuetothebroadadaptabilitytodissolvedoxygenandpH,togetherwiththehighactivityforaerobicdenitrification,thisstrainshowedthepotentialapplicationinthefuture.Keywords:Aerobicdenitrification,Urbanriver,Biologicaldenitrification,Pseudomonasputida詹吉东等:城市河道污水中好氧反硝化菌的分离鉴定与特性567http://journals.im.ac.cn/wswxtbcn氮素是我国水体污染和富营养化的主要因素之一,生物脱氮技术因其经济、有效、易操作、无二次污染等特点成为研究的热点问题之一。传统理论认为,水中氮素的去除是将氨氧化形成的硝酸盐氮在缺氧的条件下被反硝化菌类还原为氮气和N2O