ANAMMOX富集与优化停曝比对MBR-SNAD工艺的影响

中国环境科学2019,39(6)：2370~2377ChinaEnvironmentalScienceANAMMOX富集与优化停曝比对MBR-SNAD工艺的影响张凯1,张志华2,王朝朝3,李军1*,侯连刚1,梁东博1,丁凡1(1.北京工业大学建筑工程学院,城市污水深度处理与资源化利用技术国家工程实验室,北京100124；2.邢台医学高等专科学校,河北邢台054000；3.河北工程大学能源与环境工程学院,河北邯郸056038)摘要：采用膜生物反应器(MBR)研究了厌氧氨氧化细菌在富集过程中的活性变化,在启动全程自养脱氮(CANON)工艺中以恒定曝气量,通过优化停曝比实现氨氧化细菌(AerAOB)和厌氧氨氧化细菌(AnAOB)协同脱氮并且有效抑制亚硝酸盐氧化菌(NOB)的活性,然后添加有机物(乙酸钠)逐步启动同步亚硝化-厌氧氨氧化耦合异养反硝化(SNAD)工艺.结果表明,在厌氧氨氧化细菌富集过程中,通过不断缩短水力停留时间(HRT)提高进水氮负荷的方式强化厌氧氨氧化细菌活性,其平均活性由0.603mgN/(h·gVSS)提高到了8.1mgN/(h·gVSS);当恒定曝气量为50mL/min,停曝比为4:10(min:min)时,AerAOB和AnAOB对氨氮的去除量分别占总氨氮去除量的58.8%和41.2%,NOB氧化亚硝态氮的量占总硝态氮生成量的15.3%,成功抑制了NOB的活性;当C/N比为0.5,调整停曝比为4:15后,反硝化过程氮去除量占总氮去除率的20.9%,厌氧氨氧化过程氮去除量占总氮去除率的79.1%,实现了AerAOB、AnAOB和反硝化细菌(DNB)协同脱氮的目的.关键词：停曝比；同步亚硝化-厌氧氨氧化耦合异养反硝化(SNAD)；水力停留时间(HRT)；微生物活性；脱氮途径中图分类号：X703.5文献标识码：A文章编号：1000-6923(2019)06-2370-08EffectofANAMMOXenrichmentandoptimizationofnon-aeration/aerationratioinMBR-SNADprocess.ZHANGKai1,ZHANGZhi-hua2,WANGZhao-zhao3,LIJun1*,HOULian-gang1,LIANGDong-bo1,DINGFan1(1.NationalEngineeringLaboratoryofUrbanSewageAdvancedTreatmentandResourceUtilizationTechnology,TheCollegeofArchitectureandCivilEngineering,BeijingUniversityofTechnology,Beijing100124,China；2.XingtaiMedicalCollege,Xingtai054000,China；3.CollegeofEnergyandEnvironmentalEngineering,HebeiUniversityofEngineering,Handan056038,China).ChinaEnvironmentalScience,2019,39(6)：2370~2377Abstract：Theactivitychangesofanaerobicammonia-oxidizingbacteriaduringtheenrichmentprocesswasstudiedbasedonthemembranebioreactor(MBR).Completelyautotrophicnitrogenremovalovernitrite(CANON)processwasstartedupbyconstantaerationrate.Simultaneousdenitrificationofammoniaoxidizingbacteria(AerAOB)andanaerobicammoniaoxidizingbacteria(AnAOB)byoptimizingtheintermittentaerationandeffectivelyinhibitingtheactivityofnitriteoxidizingbacteria(NOB),thenthesimultaneousnitrification,anammoxanddenitrification(SNAD)processwasstartedupsuccessfullybyaddingthethecarbonsource(sodiumacetate).Theresultsshowedthatenhancedtheactivityofanaerobicammonia-oxidizingbacteriaduring