研究生Lecture荧光分析(陈老师课程)2014

2014-06-051分子荧光光谱分析技术及其在水科学研究中的应用LectureforGraduateStudents2014/06/05黄清辉TongjiUniversity讲课大纲发光现象荧光分析发展历史荧光分析基础理论叶绿素荧光分析技术及其应用有机质荧光分析技术及其应用TongjiUniversityINTRODUCTIONTOFLUORESCENCESPECTROSCOPY•Fluorescencespectroscopyisoneofthemostsensitiveofanalyticaltechniques.•Overthepast30years,progressinmedicalphysicsandbiomedicalscienceresearchhasdrivensubstantivetechnologicalandmethodologicaladvancementsinfluorescencebasedapplications.•Thesedevelopmentshaveensuredthatfluorescencespectroscopyisnowwidelyutilizedinthewatersciences.TongjiUniversity2008诺贝尔化学奖诺贝尔化学奖OsamuShimomura1960sMartinChalfie1990sRogerY.Tsien’s,todayhttp://nobelprize.orgTheDiscoveryandDevelopmentoftheGreenFluorescentProtein(GFP)TongjiUniversity050010001500200025003000197519801985199019952000200520102015年份发表篇数Presently,thereareanumberofactiveresearchgroupsworldwidethatareusingfluorescencetechniquesfortheinvestigationofaquaticsystems.ISI数据库数据库:T/A/K=Fluorescence+WaterPublicationAnal.Chem.(11261)Environ.Sci.Technol.(2913)FluorescenceTongjiUniversity发光现象发光现象Luminescenceistheemissionoflightfromanysubstance,andoccursfromelectronicallyexcitedstates.Luminescenceisformallydividedintotwocategories—fluorescenceandphosphorescence—dependingonthenatureoftheexcitedstate.Theemissionratesoffluorescencearetypically108s–1,sothatatypicalfluorescencelifetimeisnear10ns硒化镉量子点在紫外线的照射下发出荧光2014-06-052TongjiUniversityTongjiUniversityTongjiUniversityThediscoveryandcharacterizationofFluorescenceNicolásMonardes(1565),aSpanishphysicianandbotanistwhowroteonmedicinesoftheNewWorld,isusuallycreditedasbeingthefirsttodescribethebluishopalescence[蓝色乳光蓝色乳光]ofthewaterinfusionfromthewoodofasmallMexicantree.Whenmadeintocupsandfilledwithwater,apeculiarbluetingewasobserved.Coatli.patli,yoanaqujxtiloni,matlaticiniayoaxixpatli..“itisamedicine,andmakesthewaterofbluecolor,itsjuiceismedicinalfortheurine”Sahagún,FlorentineCodexVol.IIIf.266;CM-RAH,f.203v.TongjiUniversityThefirstobservationoffluorescencefromaquininesolutioninsunlightwasreportedbySirJ