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北京农学院学报2019,34(2):1—4JournalofBeijingUniversityofAgriculturehttp://bnxb.cbpt.cnki.netdoi:10.13473/j.cnki.issn.1002—3186.2019.0201菌株SDBl—2的筛选及其对垃圾渗滤液处理的应用张超1,尚润东2,刘宇璇2,杨国庆2,李璐2,施磊2,靳永胜2(1.北京市城市管理研究院,北京100028;2.北京农学院生物科学与工程学院,北京102206)摘要:【目的】为了筛选出能高效降解渗滤液中氨氮或TN的菌株。【方法】在高浓度的初始氨氮浓度条件下从北京市南宫堆肥厂垃圾渗滤液中筛选出一株能高效降解氨氮的菌株SDBl—2,通过形态观察、革兰氏染色和16S-rRNA分子鉴定,鉴定该菌株为肺炎克雷伯氏菌Citrobacterfreundii,简称SDBl—2。【结果】经研究发现,其能降解初始氨氮浓度高达2000mg/I。的人造废水。在不添加任何其他物质的条件下,该菌株对北京市某综合垃圾处理厂渗滤液中氨氮,TN和COD均能实现一定程度的降解,对同一批垃圾渗滤液进行高温高压处理后,同等条件下其最高去除率分别为51.15%、35.03%、57.45%。【结论】为环保工作者开发垃圾渗滤液处理生化工艺中氨氮、TN或COD的高效去除提供理论依据和技术支持。关键词:垃圾渗滤液;氨氮;化学需氧量;总氮中图分类号:X172文章编号:l002—3186(2019)02—0001—04文献标志码:AofstrainSDBl-2anditsapplicationintreatmentoflandfillleachateZHANGCha01,SHANGRundon92,LIUYunxuan2,YANGGuoqin92,LILu2。SHILei2,JINYongshen92(1.BeijingInstituteofUrbanManagement,Beijing102206·China;2.CollegeofBiologicalScienceandEngineering,BeijingAgricuhuralUniversity,Beijing102206,China)Abstract:Themainpollutantsoflandfillleachateareammonianitrogen,TN,COD.etc.Screeningstrainscapableofefficientlydegrad—ingammonianitrogenorTNisofgreatsignificanceforthetreatmentoflandfillleachate.Inthisstudy,astrainofSDBl—2capableofefficientlydegradingammonianitrogenwasscreenedfromthelandfillleachateofBeijingNangongCompostingPlantunderhighconcen—trationofinitialammonianitrogenconcentration。anditwasidentifiedbymorphologicalobservation。Gramstainand16S-rRNAmolec—ularidentification.ThestrainwasidentifiedasCitrobacterfreundii,referredtOasSDBl—2.IthasbeenfoundthatitcandegradeartificialwastewaterwithaninitialammonianitrogenconcentrationofuptO2000mg/L.Underthecondition0fnotaddinganyothersub—stances,thestraincanachieveacertaindegreeOfdegradationofammonianitrogen,TNandCODintheleachateofacomprehensivewastetreatmentplantinBeijing.Afterthesamebatchoflandfillleachateistreatedunderhightemperatureandhighpressure.thesameconditionsThehighestremovalrateswere51.15%,35.03%,and57.45%。respectively.ThisstudyaimstOprovidetheoreticalbasisandtechnicalsupportforenvironmentalpro
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