23-Seasonal and ecohydrological regulation of acti

23-Seasonal and ecohydrological regulation of active microbial populations involved in DOC, CO2, and CH4fluxes in temperate rainforest soilTheISMEJournalhttps://doi.org/10.1038/s41396-018-0334-3ARTICLESeasonalandecohydrologicalregulationofactivemicrobialpopulationsinvolvedinDOC,CO2,andCH4fluxesintemperaterainforestsoilDavidJ.Levy-Booth1,2●IanJ.W.Giesbrecht2,3●ColleenT.E.Kellogg1,2●ThierryJ.Heger4●DavidV.D’Amore5●PatrickJ.Keeling6●StevenJ.Hallam1●WilliamW.Mohn1Received:9February2018/Revised:12October2018/Accepted:3December2018©InternationalSocietyforMicrobialEcology2018AbstractThePacificcoastaltemperaterainforest(PCTR)isaglobalhot-spotforcarboncyclingandexport.YettheinfluenceofmicroorganismsoncarboncyclingprocessesinPCTRsoilispoorlycharacterized.WedevelopedandtestedaconceptualmodelofseasonalmicrobialcarboncyclinginPCTRsoilthroughintegrationofgeochemistry,micro-meteorology,andeukaryoticandprokaryoticribosomalamplicon(rRNA)sequencingfrom216soilDNAandRNAlibraries.SoilmoistureandpHincreased