分子生态学技术

分子生态学技术分子生态学技术介绍分子生态学技术介绍种群多样性和动态种群多样性和动态种群丰富度种群丰富度结构和功能的关系结构和功能的关系MethyleneBlueStainNon-PremoversPolyphosphateAccumulatingOrganisms(PAOs)Thetop-viewCSLMmicrographsof60-day-oldanodebiofilmformedbymixedcultureinsingle-chamberair-chatodeMFCfedwithacetate.Livecells(syto9)eDNA(DDAO)Exopolysaccharides(AlexaFluor633conjugate,concanavalinA)微生物生态系统的分子检测技术微生物生态系统的分子检测技术收集样品收集样品核酸提取和电泳核酸提取和电泳rRNAgene克隆克隆rRNAgenes分类与分类与发育分析发育分析探针技术如荧光原位杂交技术探针技术如荧光原位杂交技术(FISH)6TypeSizeLargesubunitSmallsubunitprokaryotic70S50S(5S,23S)30S(16S)eukaryotic80S60S(5S,5.8S,28S)40S(18S)Bacterial16S,23S,and5SrRNAgenesaretypicallyorganizedasaco-transcribedoperon.Theremaybeoneormorecopiesoftheoperondispersedinthegenome(forexample,Escherichiacolihasseven).ArchaeacontainseitherasinglerDNAoperonormultiplecopiesoftheoperon.The3'endofthe16SrRNA(inaribosome)bindstoasequenceonthe5'endofmRNAcalledtheShine-Dalgarnosequence.smallsubunitribosomalRNA(SSUrRNA)