Yan-Korshin2013_Chapter_QuantitationOfInteractions

Yan-Korshin2013_Chapter_QuantitationOfInteractionsOfSuQuantitationofInteractionsofSuwanneeRiverFulvicAcidwithProtonsBasedonNumericalDeconvolutionofDifferentialAbsorbanceandFluorescenceSpectraMingquanYanandGregoryV.KorshinAbstractInthischapter,weappliedanalternativeapproachthatcombinedthegenerationofdifferentialspectrawiththeirdeconvolutionthatalloweddetermin-ingtheemergenceandsignificanceoffeaturesseeninthespectroscopicdataobtainedatvaryingprotonactivities.ThebehaviorofSuwanneeRiverfulvicacid(SRFA)wasascertainedusingdifferentialabsorbancespectraandfluores-cenceexcitation�emissionmatrixes.ThepH-differentialabsorbancespectrawereprocessedvianumericdeconvolutiontodeterminethecontributionsofdiscretefeatures.DeconvolutionshowedthepresenceofsixGaussianbandsthatwereoperationallyreferredtoasA5,A4,A3,A2,A1,andA0withmaximalocatedintheorderofincreasingenergy(ordecreasingwavelengths)at2.25eV(551nm),3.24�0.02eV(383nm),3.99�0.01eV(311nm),4.52�0.03eV(275nm),5.11�0.06eV(243nm),and5.97�0.03eV(208nm).The