荧光光谱法测定痕量NOM

荧光光谱法测定痕量NOMTracingdissolvedorganicmatterinaquaticenvironmentsusinganewapproachtofluorescencespectroscopyColinA.Stedmona,*,StiigMarkagera,RasmusBrobaDepartmentofMarineEcology,NationalEnvironmentalResearchInstitute,P.O.Box358,Frederiksborgvej399,DK-4000Roskilde,DenmarkbDepartmentofDairyandFoodScience,TheRoyalVeterinaryandAgriculturalUniversity,Rolighedsvej30,DK-1958Frederiksberg,DenmarkReceived14August2002;receivedinrevisedform23January2003;accepted22April2003AbstractDissolvedorganicmatter(DOM)isacomplexandpoorlyunderstoodmixtureoforganicpolymersthatplaysaninfluentialroleinaquaticecosystems.InthisstudywehavesuccessfullycharacterisedthefluorescentfractionofDOMinthecatchmentofaDanishestuaryusingfluorescenceexcitation–emissionspectroscopyandparallelfactoranalysis(PARAFAC).PARAFACaidsthecharacterisationoffluorescentDOMbydecomposingthefluorescencematricesintodifferentindependentfluorescentcomponents.TheresultsrevealthatatleastfivedifferentfluorescentDOMfractionspresent(insignificantamounts)i