Bacterial Community Structure in the Drinking Wate

Bacterial Community Structure in the Drinking Water Microbiome Is111BacterialCommunityStructureintheDrinkingWaterMicrobiomeIsGovernedbyFiltrationProcessesAmeetJ.Pinto,†ChuanwuXi,‡andLutgardeRaskin†,*†DepartmentofCivilandEnvironmentalEngineering,UniversityofMichigan‡DepartmentofEnvironmentalHealthSciences,UniversityofMichigan*SSupportingInformationABSTRACT:Thebacterialcommunitystructureofadrinkingwatermicrobiomewascharacterizedoverthreeseasonsusing16SrRNAgenebasedpyrosequencingofsamplesobtainedfromsourcewater(amixofagroundwaterandasurfacewater),differentpointsinadrinkingwaterplantoperatedtotreatthissourcewater,andintheassociateddrinkingwaterdistributionsystem.Eventhoughthesourcewaterwasshowntoseedthedrinkingwatermicrobiome,treatmentprocessoperationslimitthesourcewater’sinfluenceonthedistributionsystembacterialcommunity.Rather,inthisplant,filtrationbydualmediarapidsandfiltersplayedaprimaryroleinshapingthedistributionsystembacterialcommunityoverseasonaltimescalesasthefiltersharboredastabl